Simultaneous immunoenzymometric assay for antibodies against human interleukin-2 and human serum albumin in rat serum
作者： Goro Kominami , Hiromi Kawamoto , Yasuo Murai , Masao Kono
DOI: 10.1080/01971529008055039
关键词: Fluorescence spectrometry 、 Horseradish peroxidase 、 Enzyme assay 、 Peroxidase 、 Antibody 、 Human serum albumin 、 Serum albumin 、 Molecular biology 、 Chemistry 、 Antigen
摘要: A simultaneous immunoenzymometric assay for anti-human interleukin-2 antibody and serum albumin in rat was developed. Two antigen-immobilized polystyrene balls were immersed a diluted sample an tube then the antibodies on made to react with horseradish peroxidase labelled anti-rat IgG same after washing. The enzyme activity of each ball measured by fluorometry. Not only sensitivity (70 ng/ml each), recovery (100-101%), precision (C.V. = 5-13%) comparable those conventional assays using one but also much more feasible mass routine assays. Thus, immunoassays can be replaced this convenient method.
sci-hub.se 参考文章(7)
S P Miller, V A Marinkovich, D H Riege, W J Sell, D L Baker, N T Eldredge, J W Dyminski, R G Hale, J M Peisach, J F Burd, Application of the MAST Immunodiagnostic System to the determination of allergen-specific IgE. Clinical Chemistry. ,vol. 30, pp. 1467- 1472 ,(1984) , 10.1093/CLINCHEM/30.9.1467
Marius Ianconescu, Simultaneous screening for two different antibodies in ELISA by combining two solid phases: Microtiter plate and falcon assay screening test system lid Journal of Immunological Methods. ,vol. 76, pp. 307- 316 ,(1985) , 10.1016/0022-1759(85)90308-4
Monique Alric, Dominique Cheyvialle, Michel Renaud, Cross-blot and cross-dot system: a high-performance system for the detection of antigen-antibody complexes on nitrocellulose. Analytical Biochemistry. ,vol. 155, pp. 328- 334 ,(1986) , 10.1016/0003-2697(86)90442-2
R. Hampl, L. Stárka, Recent advances in immunoassay of steroid hormones Trends in Analytical Chemistry. ,vol. 8, pp. 72- 75 ,(1989) , 10.1016/0165-9936(89)87022-0
Kominami Goro, Fujisaka Izumi, Yamauchi Akira, Kono Masao, A sensitive enzyme immunoassay for plasma cortisol Clinica Chimica Acta. ,vol. 103, pp. 381- 391 ,(1980) , 10.1016/0009-8981(80)90158-8
Masayuki KUROBE, Masao KOHNO, Nobuo YOSHIDA, Kyozo HAYASHI, Development of Monoclonal Antibody-Based Enzyme Immunoassay for Human Pancreatic Secretory Trypsin Inhibitor (PSTI) Suitable for Clinical Tests Journal of Clinical Biochemistry and Nutrition. ,vol. 5, pp. 173- 181 ,(1988) , 10.3164/JCBN.5.173
Regula E. Aeppli, Mario J. Bargetzi, Hans Binz, Diagnostic screening of multiple antigen-antibody reactions in a new single assay on nitrocellulose: the line immunobinding assay (LIBA). Journal of Immunological Methods. ,vol. 120, pp. 93- 98 ,(1989) , 10.1016/0022-1759(89)90293-7