The resistance of B-CLL cells to DNA damage–induced apoptosis defined by DNA microarrays

摘要: B-cell chronic lymphoid leukemia (BCLL) is a highly heterogeneous human malignancy, presumably reflecting specific molecular alterations in gene expression and protein activity that are thought to underlie the variable disease outcome. Most B-CLL cell samples undergo apoptotic death response DNA damage. However, clinically distinct aggressive subset of completely resistant vitro irradiation-induced apoptosis. We therefore addressed 2 series microarray analyses on 4 sensitive 3 compared their patterns before after stimuli. Data analysis pointed out 16 genes whose varied at least 2-fold specifically cells. validated these selected by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) 7 confirmed altered level 15 additional not included analysis. In this manner, 11 tested, 13 were found be for all samples: nuclear orphan receptor TR3, major histocompatibility complex (MHC) class II glycoprotein HLA-DQA1, mtmr6, c-myc, c-rel, c-IAP1, mat2A, fmod up-regulated, whereas MIP1a/GOS19-1 homolog, stat1, blk, hsp27, ech1 down-regulated. some cases, profile may dependent status p53. Some encode general factors but also exhibit specificities could potentially linked development malignancies (MIP1alpha, mtmr6). Taken together, our data define new markers subsets might clinical relevance.