HETEROLOGOUS POLYPEPTIDE PRODUCTION IN THE ABSENCE OF NONSENSE-MEDIATED mRNA DECAY FUNCTION

摘要: The invention relates to the discovery of a gene, NMD2, named after its role in Nonsense-Mediated mRNA Decay pathway, and protein, Nmd2p, encoded by NMD2 gene. amino acid sequence Nmd2p nucleotide gene encoding it are disclosed. is shown herein bind another protein decay Upf1p. A C-terminal fragment also Upf1p and, when overexpressed host cell, inhibits function Upf1p, thereby inhibiting nonsense-mediated pathway. methods pathway stabilize transcripts containing nonsense codon which normally would cause an increase transcript rate. Such stabilization useful for production recombinant or thereof. identifying molecules that inhibit use such treatment disorders associated with mutations.