The Saccharomyces cerevisiae RAD9, RAD17, RAD24 and MEC3 genes are required for tolerating irreparable, ultraviolet-induced DNA damage.
作者： A. G. Paulovich , Leland H. Hartwell , C. D. Armour
DOI: 10.1093/GENETICS/150.1.75
关键词: Biology 、 G2-M DNA damage checkpoint 、 Saccharomyces cerevisiae 、 DNA damage 、 DNA repair 、 Sister chromatid exchange 、 Mutagenesis 、 Mutation 、 Gene 、 Genetics
摘要: In wild-type Saccharomyces cerevisiae, a checkpoint slows the rate of progression an ongoing S phase in response to exposure DNA-alkylating agent. Mutations that eliminate regulation also confer sensitivity alkylating agents, leading us suggest that, by regulating rate, cells are either better able repair or replicate damaged DNA. this study, we determine effects mutations impair on ability excision repair-defective irreparably UV-damaged We assay survival after UV irradiation, as well genetic consequences replicating template, namely mutation and sister chromatid exchange induction. find RAD9, RAD17, RAD24, MEC3 required for UV-induced (although not spontaneous) mutagenesis, RAD9 RAD17 (but REV3, MEC3) maximal induction replication-dependent exchange. Therefore, genes only control cell cycle damage, but play role accommodating DNA damage during replication.
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Wolfram Siede, Graham C. Walker, Errol C. Friedberg, DNA Repair and Mutagenesis ,(2006)
Fred Sherman, Gerald R. Fink, James B. Hicks, Methods in yeast genetics Cold Spring Harbor Laboratory. ,(1979)
Rodney J. Rothstein, One-step gene disruption in yeast Methods in Enzymology. ,vol. 101, pp. 202- 211 ,(1983) , 10.1016/0076-6879(83)01015-0
Richard J. Reynolds, Errol C. Friedberg, Molecular Mechanisms of Pyrimidine Dimer Excision in Saccharomyces cerevisiae: Incision of Ultraviolet-Irradiated Deoxyribonucleic Acid In Vivo Journal of Bacteriology. ,vol. 146, pp. 692- 704 ,(1981) , 10.1128/JB.146.2.692-704.1981
D R Wilcox, L Prakash, Incision and postincision steps of pyrimidine dimer removal in excision-defective mutants of Saccharomyces cerevisiae. Journal of Bacteriology. ,vol. 148, pp. 618- 623 ,(1981) , 10.1128/JB.148.2.618-623.1981
Leland H. Hartwell, Macromolecule Synthesis in Temperature-sensitive Mutants of Yeast Journal of Bacteriology. ,vol. 93, pp. 1662- 1670 ,(1967) , 10.1128/JB.93.5.1662-1670.1967
E A Vallen, F R Cross, Mutations in RAD27 define a potential link between G1 cyclins and DNA replication. Molecular and Cellular Biology. ,vol. 15, pp. 4291- 4302 ,(1995) , 10.1128/MCB.15.8.4291
Roshan Christensen, Christopher W. Lawrence, Uv Mutagenesis in Radiation-Sensitive Strains of Yeast Genetics. ,vol. 82, pp. 207- 232 ,(1976) , 10.1093/GENETICS/82.2.207
R. D. Kolodner, R. A. G. Reenan, Characterization of insertion mutations in the Saccharomyces cerevisiae MSH1 and MSH2 genes: evidence for separate mitochondrial and nuclear functions. Genetics. ,vol. 132, pp. 975- 985 ,(1992) , 10.1093/GENETICS/132.4.975
A Aboussekhra, R Chanet, A Adjiri, F Fabre, Semidominant suppressors of Srs2 helicase mutations of Saccharomyces cerevisiae map in the RAD51 gene, whose sequence predicts a protein with similarities to procaryotic RecA proteins. Molecular and Cellular Biology. ,vol. 12, pp. 3224- 3234 ,(1992) , 10.1128/MCB.12.7.3224