Role of oxygen in antibody-dependent cytotoxicity mediated by monocytes and neutrophils.

摘要: The antibody-dependent cell-mediated cytoxicity (ADCC) by human monocytes and neutrophils was investigated measuring the release of 51chromate from prelabeled erythrocytes coated with immunoglobulin G. ADCC found to be positively correlated phagocytosis 51Cr-labeled postphagocytic events effector cells, activation hexose monophosphate shunt, degranulation. Exclusion oxygen incubation media halved both cell types without affectijg or Likewise, cells patients suffering chronic granulomatous disease (CGD) only half intensity normals. Inhibitors mitochondrial respiration were depressing effect ADCC. Azide, which in addition its blocking action on oxydative phosphorylation also inhibits catalase myeloperoxidase, resulted a approximately equal 40% stimulation normals but CGD patients. hydroxyl radical scavenger, mannitol, significantly depressed (P < 0.01) Azide mannitol normal when excluded. In xanthine-xanthine oxidase system, effectively lysed. This lysis inhibited catalase, superoxide dismutase, mannitol. When comparable concentrations glucose used no observed. H2O2 either alone combination azide did not lyse erythrocytes. It is suggested that partly dependent generation radicals cells.