Human leukocyte antigen-class II-negative long-term cultured human T-cell leukemia virus type-I-infected T-cell lines with progressed cytological properties significantly induce superantigen-dependent normal T-cell proliferation.

摘要: While most human T-cell leukemia virus type-I (HTLV-I)-infected T cells express abundant class II antigens, some aggressive-type adult (ATL) lose their expression. To investigate the significance of antigen HTLV-I infected cells, progressiveness HTLV-I-infected long-term cultured lines was evaluated, and then antigen-presenting capacity examined using a superantigen, staphylococcus enterotoxin B (SEB). Among cell derived from peripheral blood, HPB-ATL-T (ATL-T), HPB-ATL-2 (ATL-2) HPB-ATL-O were more progressed than Tax exclusively expressing HPB-CTL-I (CTL-I), because former deleted p16 gene (polymerase chain reaction (PCR)) strongly transcribed survivin (reverse transcriptase-PCR). Notably, interferon gamma-independent loss expression ATL-T ATL-2 found. In experiments, however, both induced SEB-dependent significant proliferation estimated by [(3)H] thymidine uptake. No II-re-expressed observed in SEB-presenting cultures indirect immunofluorescence, only minimum inhibition response anti-human leukocyte (HLA)-DR monoclonal antibody observed. These findings suggest that very effectively present SEB to less dependently on molecules. immunogenic leukemic aggressive ATL may contribute disease aggression.