Identification of a novel gene NCRMS on chromosome 12q21 with differential expression between rhabdomyosarcoma subtypes.

摘要: Rhabdomyosarcoma (RMS) is a malignant soft tissue tumor showing varying degrees of skeletal muscle differentiation. Two major histologic subtypes exist, alveolar and embryonal, each with associated molecular genetic changes. We have used Representational Difference Analysis (RDA) to compare gene expression between the two RMS identified novel NCRMS (non-coding RNA in RMS) that has increased subtype relative embryonal subtype. Multiple alternatively spliced forms were through library screening, RACE, comparison human expressed sequence tags (ESTs). Northern blot analysis indicated transcript size be 1.25 kb RMS. There was no homology any known genes GenBank, but extensive ESTs from various species. Comparison genomic sequences at least 11 exons mapping chromosomal region 12q21. Differential noted types. Since possesses limited potential for protein coding, yet conserved different species, it likely functional non-coding RNA. Known its proximity include myogenic regulators Myf5 Myf6, growth factor Igf1, another differentially (ATP2B1) isolated by RDA.