Identification of Amelotin‐ and ODAM‐interacting enamel matrix proteins using the yeast two‐hybrid system

摘要: Holcroft J, Ganss B. Identification of Amelotin- and ODAM-interacting enamel matrix proteins using the yeast two-hybrid system. Eur J Oral Sci 2011; 119 (Suppl. 1): 301–306. © 2011 Sci The formation dental is a prototype functional tissue development through biomineralization. Amelotin (AMTN) recently discovered secreted protein predominantly expressed during maturation stage formation. It accumulates in basal lamina-like structure at interface between ameloblasts mineral it co-localizes with another described protein, odontogenic ameloblast-associated (ODAM). The purpose this study was to determine whether AMTN ODAM bind each other and/or well-established proteins. coding sequences all were cloned into appropriate vectors GAL4-based Matchmaker Gold Yeast Two-Hybrid System. growth cells on selective media color induction used as indicators for reporter gene expression protein–protein interactions combinations prey bait constructs. We found that interacts itself ODAM, but not amelogenin (AMEL), ameloblastin (AMBN), or enamelin (ENAM). Using bait, interaction confirmed. Furthermore, AMBN, well weakly AMEL ENAM. propose model where distinct their are involved defining microstructure surface.