Oxygen sensor nanoparticles for monitoring bacterial growth and characterization of dose–response functions in microfluidic screenings

摘要: We are presenting a microfluidic droplet-based system for non-invasive, simultaneous optical monitoring of oxygen during bacterial cultivation in nL-sized droplets using ~350 nm nanobeads made from polystyrene and doped with the NIR-emitting probe platinum (II) 5, 10, 15, 20-meso-tetraphenyltetrabenzoporphyrin (PtTPTBP). Data were readout by two-channel micro flow-through fluorimeter photometer. The time-resolved miniaturized multi endpoint detection was applied to simultaneously sense dissolved oxygen, cellular autofluorescence, cell density segments. Two strains studied that resistant heavy metal ions, viz. Streptomyces acidiscabies E13 Psychrobacillus psychrodurans UrPLO1. study has two main features it demonstrates (a) possibility monitor changes partial pressure metabolic activity different cultures inside droplets, (b) efficiency techniques along multi-parameter sensing highly resolved microtoxicological screenings aquatic systems.