Measurement of Tumor Cytolysis by Macrophages
作者： Mónica Escórcio‐Correia , Thorsten Hagemann
DOI: 10.1002/0471142735.IM1418S92
关键词: Biology 、 Cytolysis 、 Molecular biology 、 Annexin 、 Cytotoxicity 、 Programmed cell death 、 Cell culture 、 Cytotoxic T cell 、 Cell 、 Fluorescence staining
摘要: This unit describes two different protocols for the measurement of tumor cytolysis by macrophages. Traditionally, cytotoxicity assays have relied on use radioactive isotopes. In Basic Protocol 1, cytotoxic activity is measured release into culture supernatant a radioisotope that had been incorporated target cell and released upon death. poses problem some lines in which spontaneous isotope occurs absence effector cytotoxicity. 2, nonradioactive approach used to measure relies fluorescence staining cells with cell-death markers. It also provides obvious advantage avoiding hazardous materials. Curr. Protoc. Immunol. 92:14.18.1-14.18.11. © 2011 John Wiley & Sons, Inc. Keywords: macrophages; cytolysis; tumor; annexin V; 7-AAD; CFDA-SE; [111In]oxine
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