Sparc-like protein 1 is a new marker of human glioma progression.

摘要: High-grade gliomas (glioblastomas) are the most common and deadly brain tumors in adults, currently with no satisfactory treatment available. Apart from de novo glioblastoma, it is accepted that these malignancies mainly progress lower grade glial tumors. However, molecular entities governing progression of poorly understood. Extracellular membrane proteins key biomolecules found at cell-to-cell communication interface hence a promising proteome subpopulation could help understand development glioma. Accordingly, current study aims identifying new protein markers human glioma progression. For this purpose, we used generated orthotopically T98G U373 cells nude mice. This setup allowed also to discriminate origin, namely, (tumor) or mouse (host). were selectively purified using biotinylation followed by streptavidin affinity chromatography. Isolated digested then identified quantified employing 2D- nano-HPLC−MS/MS analysis. A total 23 27 up- regulated extracellular models, respectively. Approximately two-thirds predominantly produced tumor, whereas remaining appeared be overexpressed host tissue. Following extensive validation, have focused our attention on sparc-like 1. was further investigated immunohis- tochemistry large collection samples different grades. The results showed 1 expression correlates grade, suggesting possible role for malignancy.