Robust multiparametric assessment of cellular senescence.
作者： Clara Correia-Melo , Diana Jurk , João F. Passos
DOI: 10.1007/978-1-62703-239-1_27
关键词: Cell biology 、 In vivo 、 Immunofluorescence 、 Suppressor 、 Biology 、 Ki-67 、 Senescence 、 DNA damage 、 Nucleus 、 Proliferating cell nuclear antigen
摘要: Cellular senescence, the irreversible loss of replicative capacity, is both a tumor suppressor mechanism and contributor to age-related tissue function. However, role cellular senescence in vivo has been unclear, mostly because absence markers specific enough identify state (senescent or proliferating) individual cells tissues. Recently, we have tested robustness multiple candidate by comparing them dynamic stimulation model, which estimates fraction senescent with high precision. We found that proliferation Ki67 PCNA combined density DNA damage foci (>5 γH2AX per nucleus) was best quantitative indicator senescence. In this chapter, describe protocols for dual immunofluorescence-based quantification Ki67/PCNA fixed paraffin-embedded
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