Taurine prevents myocardial ischemia/reperfusion-induced oxidative stress and apoptosis in prolonged hypothermic rat heart preservation

摘要: Taurine (2-aminoethanesulfonic acid) is a potent antioxidant and inhibits cell apoptosis in ischemic reperfusion injury. In this study we evaluated whether addition of taurine to St. Thomas' cardioplegic solution enhances its myocardial protective effects prolonged hypothermic heart preservation rats. Hearts isolated from male Sprague–Dawley rats were mounted on Langendorff apparatus estimate baseline cardiac function, then arrested stored solution, with (10 mM; group, n = 8) or without (control 8), for 6 h at 4°C. After storage, the hearts reperfused rate (HR), coronary flow (CF), left ventricular developed pressure (LVP), positive maximum developing (max LV dp/dt) measured. The tissue was examined immunohistochemically determining DNA oxidative stress apoptosis. Compared control groups, recovery LVP (P < 0.001), max dp/dt 0.001) significantly enhanced, whereas glutamic oxaloacetic transaminase 0.01), lactate dehydrogenase 0.05), creatine phosphate kinase 8-hydroxy-2′-deoxyguanosine index caspase-3 mRNA expression percentage TUNEL-positive cardiomyocytes 0.05) reduced group. Addition improved function rat by suppressing