Fast estimation of ATP/ADP ratio as a special step in pharmacological and toxicological studies using the cell-free translation systems
作者： Dmitrij A. Kuznetsov , Alexandre V. Govorkov , Nikolay V. Zavijalov , Taniyousha M. Sibileva , Volker Richter
DOI: 10.1016/0165-022X(86)90008-4
关键词: High-performance liquid chromatography 、 Lysis 、 Pactamycin 、 ATP–ADP translocase 、 Cycloheximide 、 Reticulocyte 、 Elution 、 Chromatography 、 Barbituric acid 、 Chemistry 、 Biophysics 、 Biochemistry
摘要: Abstract We have developed a simple and effective reversed-phase HPLC procedure for rapid estimation of the ATP/ADP ration in cell-free translation system containing creatine kinase. Analysis acetone-extractable pool derived from reticulocyte lysate was carried out by automatic chromatography on S5CN-ODS stationary phase using linear 10–65% pyridine elution gradient formed basis methanol/water (9:1, v/v) mobile phase. this method used to detect characterize inhibition induced considerable suppression ATP resynthesis vitro. It shown that methyl mercury, unlike cycloheximide, pactamycin, CCl 4 barbituric acid, exerts inhibitory effect regeneration system.
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