Antibody-dependent cell-mediated cytotoxicity in human cancer: Characterization of patient leukocyte activity and treatment effects

摘要: Antibody-dependent cell-mediated cytotoxicity (ADCC), medicated by peripheral blood Hypaque-Ficoll separated mononuclear cells, was studied in humans using chicken erythrocytes (CRBC) incubated a 1:1200 dilution of rabbit anti-CRBC and human B (HRBC) incubation 1:20 isoantibody. At the optimal target effector ratio 3:1, ADCC to both CRBC HRBC significantly higher than normal 27 lung cancer, 18 malignant melanoma, seven colon cancer patients, but not 20 breast patients. Chemotherapy (single-agent or combination) 12 patients did effect vitro suppressed targets after only four five days therapy vivo (ADCC CRBC, 47.4 24.1% lysis: HRBC, 48.1 16.3% lysis). Immunotherapy with intravenous (IV) corynebacterium parvum IV methanol extraction residue BCG (MER) boosted within first dose. It found that completely absent three cases active hairy cell leukemia present two remission. The showed an age-dependent increase 51 subject This observed for CRBC. mediated mainly Fc-receptor-positive, nonadherent, small lymphocyte, entirely adherent monocyte. correlate H3 thymidine incorporation cultured without stimulation either one days. also number residual granulocytes suspensions. Measurement is useful method characterizing host defense disease its modification therapy.