Identification of novel blood proteins specific for mammalian hibernation.
作者： N Kondo , J Kondo
DOI: 10.1016/S0021-9258(18)48519-5
关键词: Chipmunk 、 Hibernation 、 Peptide sequence 、 Biochemistry 、 Ground squirrel 、 Rodent 、 Amino acid 、 Blood proteins 、 Biology 、 Gel electrophoresis
摘要: Mammalian hibernation is a unique physiological adaptation that allows the sustainment of life under extremely low body temperatures. In chipmunk, we found four proteins related specifically to hibernation. These started diminish in concentration blood before and disappeared during reappeared as ceased remained nonhibernation. The complete or partial amino acid sequences showed three (27-, 25-, 20-kDa) were previously unknown, whereas another (55-kDa) highly homologous with alpha 1-antitrypsin. novel are homologous, indicating they family. NH2-terminal regions these proteins, collagen-like sequence present, their COOH-terminal regions, two sequences, Ser-Ala-Phe-Ala-Val-Lys Val-Trp-Leu-Glu, conserved. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis nonreducing conditions permeation chromatography denaturating revealed form 140-kDa complex plasma fraction. detected hibernator, ground squirrel, but not rodent nonhibernators, namely tree squirrels rats. present finding first identification hibernation-specific protein. presence specific hibernators suggests involvement genetic factors control provide valuable tools for understanding molecular mechanisms mammalian
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