Plasma membrane Ca2+-ATPase mRNA expression in murine hepatocarcinoma and regenerating liver cells.
作者： Blanca Delgado-Coello , Juan Santiago-García , Angel Zarain-Herzberg , Jaime Mas-Oliva
关键词: Plasma membrane Ca2+ ATPase 、 Alternative splicing 、 Gene isoform 、 Calcium 、 Extracellular 、 Calcium pump 、 Cytoplasm 、 Biology 、 ATPase 、 Molecular biology
摘要: The plasma membrane calcium ATPase (PMCA) is an ubiquitous enzyme that extrudes from the cytoplasm to extracellular space. Four PMCA genes through alternative splicing produce a large diversity of isoforms this enzyme. We reported previously contained in AS-30D hepatocarcinoma cells showed significant differences activity comparison normal and regenerating liver. In present study we investigate if difference could be related differential expression mRNAs encoding different PMCA. Using RT-PCR found variants 1b, 1x, 4b are expressed all liver samples. hepatoma AS-30 at 2 days regeneration express low amounts 2w, 4x, do not 4a, 4d 4z. Fetal neonatal 4a 4d, but they 4x Immunoblot analysis higher ratio ATPase/total protein Our results suggest Ca2+-ATPase kinetic pattern observed by us cells, least partially explained changes mRNA several
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