Plasma membrane calcium ATPase isoform 3 expression in single cells isolated from rat liver

摘要: The plasma membrane Ca(2+)-ATPase (PMCA) located in the hepatocyte is a controversial molecule itself since it displays different features to those regarded as canonical for P-type Ca(2+)-ATPases, and from which transcript expression well catalytic activity continues be under active investigation. Our aim this study was explore at first glance, pmca isoform distribution using isolated parenchymal non-parenchymal cells rat liver tissue. Expression of transcripts analyzed fresh or cell-enriched culture preparations, confirming pmca1 pmca4 housekeeping isoforms all cell types studied (hepatocytes, Kupffer cells, stellate cells). However, time we show pmca3 edited two sites both hepatocytes cells. Interestingly, employing demonstrate specific pmca3e previously considered nearly exclusive excitable tissues. Real-time PCR quantification shows significant decrease cultured hepatic comparison with presence pmca2 along suggests that high affinity are relevant adequate management calcium tissue, particularly when become activated by diverse stimuli.