The novel serine protease tumor-associated differentially expressed gene-14 (KLK8/Neuropsin/Ovasin) is highly overexpressed in cervical cancer☆

摘要: Abstract Objective Serine proteases are redundant enzymes implicated in the extracellular modulation required for tumor growth and invasion. Tumor-associated differentially expressed gene-14 (TADG-14) is a novel transmembrane serine protease recently reported by our group to be highly overexpressed ovarian carcinomas. The goal of this study was investigate frequency expression TADG-14 gene human cervical tumors. Study design evaluated 19 cancer cell lines (11 primary 8 established lines) as well normal keratinocyte cultures reverse transcriptase polymerase chain reaction. In addition, validate data at protein level, immunohistochemistry on paraffin-embedded tissue from which all 11 were established. Results found 82% (9/11) 87% (7/8) transcriptase–polymerase Expression squamous tumors 100% (6/6), whereas 60% (3/5) adenocarcinomas TADG-14. contrast, none control (n = 4) or flash frozen biopsy specimens Immunohistochemistry staining confirmed cells its absence epithelial cells. Conclusion Cervical high level TADG-14, suggesting that may play an important role invasion metastasis. Because appears only abundance contains secretion signal sequence, secreted, it prove useful diagnostic tool early detection recurrent/persistent after standard treatment molecular target therapy.