Bivalent IgY antibody constructs for diagnostic and therapeutic applications
作者： Kerstin Greunke , Edzard Spillner , Reinhard Bredehorst , Thomas Dr. Grunwald
DOI:
关键词: Molecular biology 、 Hinge region 、 Biology 、 Bivalent (genetics) 、 Recombinant DNA 、 Disulfide bond 、 Monoclonal antibody 、 Molecular stability 、 Antibody 、 Heavy chain
摘要: This invention relates to the field of recombinant antibody technology. It provides novel IgY constructs for diagnostic and therapeutical applications. The bivalent display a heterotetrameric or homodimeric format stabilized by disulfide bonds. constant heavy chain domains C H 2-C 4 are partly completely avian origin, whereas V , L 1 as well hinge region may be origin derived from any other species. allows combine advantages antibodies with those established mammalian monoclonal antibodies. comprising nonglycosylated allow reduce unwanted interactions C-type lectins, e.g., in human sera. Furthermore, chimeric containing provide higher molecular stability than acidic conditions and, thereby, especially suited peroral therapeutic
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