Passive cutaneous anaphylaxis in the chicken: biological fractionation of the mediating antibody population

摘要: The studies reported here demonstrated that adult chickens, when immunized with bovine serum albumin, respond the production of antibodies which will mediate passive cutaneous anaphylactic (PCA) reactions in 10-day-old chicks but not adults. This difference is at least part due to differences histamine sensitivity between and molecule responsible for this PCA activity was indistinguishable from predominant 7S chicken immunoglobulin antigenically by a combination gel filtration on Sephadex G-200 ion exchange chromatography DEAE-cellulose. However, apparently biologically separable portions population since has never been elicited immunoglobulins derived yolk. On basis stability denaturing treatments short tissue fixation time periods, PCA-mediating appear resemble γG1 homocytotrophic mice guinea-pigs instead IgE-type reagins found some mammals. Therefore, be subpopulation immunoglobulins, distinguishable its inability pass into egg yolk standard chemical immunochemical criteria.