Allele-specific replication timing of imprinted gene regions

摘要: SEVERAL lines of evidence suggest that the paternal and maternal genomes may have different expression patterns in developing organism1 this has been confirmed by identification endogenous genes are parentally imprinted mouse2–5. Little is known about precise mechanisms involved process, but structural differences between two alleles must somehow provide cis-acting signals for directing parental-specific transcription. Cell-cycle replication time one parameter shown to be associated with both tissue-specific gene expression6,7 allele-specific transcription X chromosomes female cells8. For reason we examined timing chromosomal regions containing Igf2, Igf2r, H19 Snrpn mouse. At all these sites, their corresponding positions human genome, homologous replicate asynchronously it always allele early-replicating. Thus appear embedded large DNA domains differential patterns, which a imprint parental identity.