Pathology-targeted cell delivery via injectable micro-scaffold capsule mediated by endogenous TGase.
作者： Chunxiao Qi , Yaqian Li , Patrick Badger , Hongsheng Yu , Zhifeng You
DOI: 10.1016/J.BIOMATERIALS.2017.02.021
关键词: In vivo 、 Cell therapy 、 Tissue transglutaminase 、 Cell 、 Mesenchymal stem cell 、 Biomedical engineering 、 Anoikis 、 Cancer research 、 Medicine 、 Regenerative medicine 、 Ex vivo
摘要: Targeted cell delivery to lesion sites via minimally invasive approach remains an unmet need in regenerative medicine endow satisfactory therapeutic efficacy and minimized side-effects. Here, we rationally designed a pathology-targeted strategy leveraging injectable micro-scaffolds as cell-loading capsule endogenous tissue transglutaminase (TGase) at site adhesive. Up-regulated TGase post-liver injury catalyzed chemical bonding between the glutamine lysine residues on liver surface both ex vivo vivo, facilitating sufficient adhesion pathological liver. Upon intraperitoneal injection, Mesenchymal Stem Cell-loaded capsules, exhibiting protection from shear-induced damage post-transplantation anoikis, adhered CCl4-treated with hundred-fold improvement targeting efficiency (70.72%) compared free-cell which dramatically improved mice survival (33.3% vs. 0% for therapy) even low-dosage treatment. This unique widely-applicable mechanism hold great promise transforming therapy refractory diseases.
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