Preformed gelatin microcryogels as injectable cell carriers for enhanced skin wound healing.

摘要: Abstract Wound dressings of cell-laden bulk hydrogel or scaffold were mainly applied for enhanced cell engraftment in contrast to free injection. However, dressing cells laden biomaterials on wound surface might not effectively and timely exert functions deep chronic wounds where insufficient blood supply exists. Previously, we developed injectable gelatin microcryogels (GMs) which could load delivery therapy. In this study, biological changes human adipose-derived stem (hASCs) GMs compared varied aspects with traditional two dimensional (2D) culture, such as phenotype markers, stemness genes, differentiation, secretion growth factors, apoptosis memory by FACS, QRT-PCR ELISA, that demonstrated the priming effects upregulation genes improved factors hASCs potential augmented healing. a full-thickness skin model nude mice, multisite injection hASCs-laden significantly accelerate healing Bioluminescence imaging protein analysis indicated retention multiple factors. Our study suggests primed 3D micro-niches represent new methodology only benefit recovery bed but also play direct basal layer enhancement. Injectable facile approach potentially provide minimally-invasive therapeutic strategy refractory diabetic ulcer radiative wound. Statement significance This work type elastic micro-scaffold prime hMSCs Due injectability GMs, cellular simply realize accelerating process superior The features MSCs has been thoroughly characterized during culture (i.e. proliferation, characterization differentiation induced GMs). Multiple methods bioluminescent imaging, immunohistochemistry, immunofluorescence, qRT-PCR, ELSA western blot used assess vivo results between groups.