Eruberin A, a Natural Flavanol Glycoside, Exerts Anti-Fibrotic Action on Pancreatic Stellate Cells.

摘要: Background: Eruberin A (2, 3-dehydroflavonoid), a flavanol glycoside isolated from Pronephrium penangianum, has been used as blood-nourishing folk medicine for centuries; however, it indeed possesses variety of other health-promoting benefits including anti-fibrotic bioactivity. Activation pancreatic stellate cells (PSCs) is the key initiating step in fibrosis, which characteristic feature associated with chronic pancreatitis and adenocarcinoma. Methods: The effect eruberin underlying mechanisms its action LTC-14 cells, retained essential characteristics morphological features primary PSCs, were examined by means real-time polymerase chain reactions, Western blotting immunostaining. Results: application (20 µg/ml) effectively inhibited expression levels fibrotic mediators namely alpha-smooth muscle actin, fibronectin type I-collagen, so sonic hedgehog signaling pathway components post transforming growth factor-beta (5 ng/ml) stimulation. treatment also led to notable decrease activation nuclear factor-kappaB (NF-κB) phosphorylation phosphoinositide 3-kinase (PI3K)/serine-threonine kinase (AKT). Conclusion: Our results demonstrated that significantly suppressed we suggest mechanism was an attenuation PI3K/AKT/NF-κB pathway.