Growth hormone and Pit-1 mRNA detection using reverse transcription-polymerase chain reaction in adult and developing Ames dwarf mice.
作者： DAVID L. HURLEY , PATRICK W. WOJTKIEWICZ , CAROL J. PHELPS
DOI: 10.1016/B978-0-12-571150-0.50034-2
关键词: Molecular biology 、 Dwarfism 、 Messenger RNA 、 Growth hormone 、 Reverse transcription polymerase chain reaction 、 Gene expression 、 Genetics 、 Transcription (biology) 、 Biology
摘要: Publisher Summary This chapter provides an overview of the study to investigate use RT-PCR detect mRNAs relevant elucidating patterns gene expression in developing Ames dwarf pituitary. The for GH mRNA a rapid and reliable means determining dwarfism at ages before body-size differences become apparent. presents method that classification dwarfs vs normals is 100% correct animals could be identified by size. not present pituitary within three days initiation transcription normal mouse mutation appears involved blockage transcription, implicating involvement Pit-1 effects mutation. assays confirmed absence detectable 30 age or older. A discussed shows even seven age.
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