Granulocyte superoxide anion and elastase release during cardiopulmonary bypass.

摘要: : Cardiopulmonary bypass (CPB) is known to induce several pathogenic responses in cardiovascular surgery. To explore leukocyte activation during PCB, we investigated superoxide anion (O2-) production by granulocytes 6 patients undergoing aortocoronary O2- was determined with chemiluminescence amplified a cypridina luciferin analogue. Granulocytes collected from the blood arterial site of CPB circuit were stimulated phorbol myristate acetate, n-formyl-methionyl-leucyl-phenylalanine, and opsonized zymosan. All stimulators failed disclose significant difference between magnitude after CPB. However, complement detected, plasma level granulocyte elastase increased gradually This discrepancy unchanged increase inflammatory mediators including may be due sequestration activated extravascular tissues. Namely, it highly likely that responsible for sequestered remained outside circulation.