Simultaneous synthesis and biological applications of DNA fragments: an efficient and complete methodology.
作者： Ronald Frank , Andreas Meyerhans , Konrad Schwellnus , Helmut Blöcker
DOI: 10.1016/0076-6879(87)54079-4
关键词: Sequence (biology) 、 Computational biology 、 Combinatorial chemistry 、 Biology 、 Fragment (logic) 、 Mutagenesis (molecular biology technique) 、 Oligonucleotide 、 DNA 、 Flexibility (engineering) 、 Gene 、 Nucleic acid
摘要: … analysis) the products are purified by short column chromatography on silica gel 60, with a … The products are purified by short column chromatography on silica gel 60 with a stepwise …
sci-hub.se 参考文章(28)
Hans W.D. Matthes, W. Martin Zenke, Thomas Grundström, Adrien Staub, Marguerite Wintzerith, Pierre Chambon, Simultaneous rapid chemical synthesis of over one hundred oligonucleotides on a microscale. The EMBO Journal. ,vol. 3, pp. 801- 805 ,(1984) , 10.1002/J.1460-2075.1984.TB01888.X
Christopher J. Welch, Jyoti Chattopadhyaya, Kjell Undheim, Ing-Marie Åstrand, Jan-Eric Berg, Thomas W. Dingle, Richard Vaughan Williams, Ramanathan Mahedevan, 3-N-Acyl Uridines: Preparation and Properties of a New Class of Uracil Protecting Group. Acta Chemica Scandinavica. ,vol. 37b, pp. 147- 150 ,(1983) , 10.3891/ACTA.CHEM.SCAND.37B-0147
B.C. Froehler, M.D. Matteucci, Dialkylformamldines: depurination resistant N6-protecting group for deoxyadenosine Nucleic Acids Research. ,vol. 11, pp. 8031- 8036 ,(1983) , 10.1093/NAR/11.22.8031
Lutz Grotjahn, Ronald Frank, Helmut Blöcker, Ultrafast sequencing of oligodeoxyribonucleo tides by FAB-mass spectrometry Nucleic Acids Research. ,vol. 10, pp. 4671- 4678 ,(1982) , 10.1093/NAR/10.15.4671
N.D. Sinha, J. Biernat, J. McManus, H. Köster, Polymer support oligonucleotide synthesis XVIII1.2): use of β-cyanoethyi-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product Nucleic Acids Research. ,vol. 12, pp. 4539- 4557 ,(1984) , 10.1093/NAR/12.11.4539
V. Sgaramella, H.G. Khorana, CXII. Total synthesis of the structural gene for an alanine transfer RNA from yeast. Enzymic joining of the chemically synthesized polydeoxynucleotides to form the DNA duplex representing nucleotide sequence 1 to 20 Journal of Molecular Biology. ,vol. 72, pp. 427- 444 ,(1972) , 10.1016/0022-2836(72)90155-6
Johann Ott, Fritz Eckstein, Filter disc supported oligonucleotide synthesis by the phosphite method Nucleic Acids Research. ,vol. 12, pp. 9137- 9142 ,(1984) , 10.1093/NAR/12.23.9137
Stefan V. Nilsson, Göran Magnusson, Sealing of gaps in duplex DNA by T4 DNA ligase. Nucleic Acids Research. ,vol. 10, pp. 1425- 1437 ,(1982) , 10.1093/NAR/10.5.1425
Mary Lynn Duckworth, Michael J. Gait, Philip Goelet, Guo Fang Hong, Mohinder Singh, Richard C. Titmas, Rapid synthesis of oligodeoxyribonucleotides VI. Effident, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route Nucleic Acids Research. ,vol. 9, pp. 1691- 1706 ,(1981) , 10.1093/NAR/9.7.1691
Hansen M. Hsiung, Roland Brousseau, Joseph Michniewicz, Saran A. Narang, Synthesis of human insulin gene. Part I. Development of reversed-phase chromatography in the modified triester method. Its application in the rapid and efficient synthesis of eight deoxyribo-oligonucleotides fragments constituting human insulin A DNA Nucleic Acids Research. ,vol. 6, pp. 1371- 1385 ,(1979) , 10.1093/NAR/6.4.1371