Dietary Free and Esterified Cholesterol Absorption in Cholesterol Esterase (Bile Salt-stimulated Lipase) Gene-targeted Mice

摘要: The involvement of pancreatic cholesterol esterase (bile salt-stimulated lipase) in absorption through the intestine has been controversial. We have addressed this issue by using homologous recombination embryonic stem cells to produce mice lacking a functional gene. Cholesterol knockout and their wild type counterparts were fed bolus dose [3H]cholesterol trace amount [β-14C]sitosterol gavage. ratio two radiolabels excreted feces over 24-h period was found be similar control esterase-null mice. Similar results observed when radiolabeled sterols supplied an emulsion with phospholipid triolein or lipid vesicles phosphatidylcholine. between regardless whether animals low fat diet high fat/high diet. rate appearance serum gene-targeted paralleled that animals. In contrast these results, experiments performed [3H]cholesteryl oleate instead [3H]cholesterol, higher 3H radiolabel dramatically less detected comparison Serum levels not significantly different either atherogenic These indicate is responsible for mediating intestinal cholesteryl esters but does play primary role free absorption.