Overexpression of the immediate-early genes Egr1, Egr2, and Egr3 in two strains of rodents susceptible to audiogenic seizures

摘要: Genetic animal models of epilepsy are an important tool for further understanding the basic cellular mechanisms underlying epileptogenesis and developing novel antiepileptic drugs. We conducted a comparative study gene expression in inferior colliculus, nucleus that triggers audiogenic seizures, using two models, Wistar rat (WAR) genetic seizure hamster (GASH:Sal). For this purpose, both were exposed to high intensity auditory stimulation, 60min later, colliculi collected. As controls, intact rats Syrian hamsters subjected stimulation tissue preparation protocols identical those performed on experimental animals. Ribonucleic acid was isolated, microarray analysis comparing stimulated WAR showed genomic profile these animals displayed significant (fold change, |FC|≥2.0 p<0.05) upregulation 38 genes downregulation 47 genes. Comparison profiles between control GASH:Sal revealed 10 5 Among common altered we identified zinc finger immediate-early growth response Egr3. The Egr3 protein is transcription factor induced by distinct stress-elicited factors. Based immunohistochemistry, expressed cochlear complex, hippocampus as well lymphoma tumors GASH:Sal. Our results support overexpression might contribute neuronal viability development stress associated with seizures. This article part Special Issue entitled "Genetic Reflex Epilepsies, Audiogenic Seizures Strains: From Experimental Models Clinic".