Molecular and neurochemical substrates of the audiogenic seizure strains: The GASH:Sal model.

摘要: article Purpose:Animalmodelsofaudiogenicepilepsyareusefultoolstounderstandthemechanismsunderlyinghuman reflex epilepsies. There is accumulating evidence regarding behavioral, anatomical, electrophysiological, and genetic substrates of audiogenic seizure strains, but there are still aspects concerning their neurochemical basis that remain to be elucidated. Previous studies have shown the involved γ-amino butyric acid (GABA) in seizures. The aim our research was clarify role GABAergic system generation epileptic seizures seizure-prone hamster (GASH:Sal) strain. Material methods: We studied K + /Cl � cotransporter KCC2 β2-GABAA-type receptor (GABAAR) β3-GABAAR subunit expressions GASH:Sal both at rest after repeated sound-induced dif- ferent brain regions using Western blot technique. also sequenced coding region for gene wild- type hamsters. Results: Lower expressionof KCC2protein wasfoundinGASH:Salwhencomparedwith controls atrest inseveral areas: hippocampus, cortex, cerebellum, hypothalamus, pons-medulla, mesencephalon. Repeated in- duction caused a decrease protein content inferior colliculus hippocampus an increase pons-medulla. When compared controls, basal β2-GABAAR overexpressed colliculus, mesencephalon, whereas β3 levels were lower increased β2 hypothalamus hypothalamus. No differences gene-coding found between wild-type Conclusions: These data indicate functioning impaired strain, seem aggravate this dysfunction. results potential clinical relevance support validity employing strain as model study neurochemistry epilepsy.