Oxygen- and NssR-dependent Globin Expression and Enhanced Iron Acquisition in the Response of Campylobacter to Nitrosative Stress

摘要: Pathogenic bacteria experience nitrosative stress from NO generated in the host and nitrosating species such as S-nitrosoglutathione. The food-borne pathogen Campylobacter jejuni responds by activating gene expression a small regulon under control of NO-sensitive regulator, NssR. Here, we describe full extent S-nitrosoglutathione response using transcriptomic proteomic analysis batch- chemostat-cultured C. jejuni. In addition to NssR regulon, which includes two hemoglobins (Cgb Ctb), identify more than 90 other up-regulated genes, notably those encoding heat shock proteins involved oxidative tolerance iron metabolism/transport. Up-regulation subset these including cgb, is also elicited NO-releasing compounds. Mutation iron-responsive regulator Fur results insensitivity growth NO, suggesting that derepression iron-regulated genes augmentation acquisition physiological damage. We effect oxygen availability on tolerance; cells cultured at higher rates diffusion have elevated levels hemoglobins, are resistant inhibition both respiration, consume rapidly. mediated Thus, detoxification catalyzed Cgb possibly Ctb, mounts an extensive response. suggest respiration may increase for synthesis function.