Validation and Marker-Assisted Selection of Stem Rust Resistance Gene Sr2 in Indian Wheat Using Gel-Based and Gel-Free Methods
作者： Gautam Vishwakarma , Ravi Prakash Sanyal , Abhijit Shitre , D. A. Gadekar , Ajay Saini
DOI: 10.1007/S12892-017-0083-0
关键词: Genotype 、 Ug99 、 Genetics 、 Marker-assisted selection 、 Biology 、 Locus (genetics) 、 Allele 、 Gene 、 Microsatellite 、 Stem rust
摘要: Stem rust resistance gene Sr2 is an important slow-rusting which has provided against stem for many years. The durable all pathogens including the Ug99 group of races. It shows recessive inheritance and linked with two phenotypic markers, Pseudo Black Chaff (PBC) High Temperature-Induced Seedling Chlorosis (HTISC). However, direct screening as well marker-based selection difficult due to effect environmental factors genetic background. Microsatellite marker Xgwm533 useful rapid large populations. Here we report validation use 21 wheat lines 24 genotypes presence gene. Furthermore, also a new, smaller allele (97 bp) locus in few that was confirmed by sequencing. We demonstrate new SYBR green dye, melt-curve/profile-based assay convenient locus, where different alleles can be differentiated gel-free manner.
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