Episomal Vectors Rapidly and Stably Produce High-Titer Recombinant Retrovirus
作者： Todd M. Kinsella , Garry P. Nolan
DOI: 10.1089/HUM.1996.7.12-1405
关键词: Titer 、 Helper virus 、 Retrovirus 、 Transfection 、 DNA replication 、 Molecular biology 、 Virology 、 Viral replication 、 Recombinant DNA 、 Biology 、 Virus 、 Molecular medicine 、 Genetics
摘要: The nuclear replication and retention functions of the Epstein-Barr virus (EBV) have been utilized here to maintain retroviral constructs episomally within human cell-based packaging lines. These hybrid EBV/retroviral are capable producing helper-free recombinant retrovirus as soon 48 hr for at least 30 days after transfection into 293T-based ecotropic and/or amphotropic cells. Viral titers greater than 10(7) TU/ml were obtained puromycin selection transfected This episomal approach production circumvents some limitations inherent in transient chromosomally stable producer systems, affording reproducibly rapid, large-scale, stable, high-titer production.
liebertonline.com参考文章(40)
MH Finer, TJ Dull, L Qin, D Farson, MR Roberts, kat: A high-efficiency retroviral transduction system for primary human T lymphocytes Blood. ,vol. 83, pp. 43- 50 ,(1994) , 10.1182/BLOOD.V83.1.43.43
N R Landau, D R Littman, Packaging system for rapid production of murine leukemia virus vectors with variable tropism. Journal of Virology. ,vol. 66, pp. 5110- 5113 ,(1992) , 10.1128/JVI.66.8.5110-5113.1992
A Adams, Replication of latent Epstein-Barr virus genomes in Raji cells. Journal of Virology. ,vol. 61, pp. 1743- 1746 ,(1987) , 10.1128/JVI.61.5.1743-1746.1987
P J Krysan, S B Haase, M P Calos, Isolation of human sequences that replicate autonomously in human cells. Molecular and Cellular Biology. ,vol. 9, pp. 1026- 1033 ,(1989) , 10.1128/MCB.9.3.1026
S S Heinzel, P J Krysan, M P Calos, R B DuBridge, Use of simian virus 40 replication to amplify Epstein-Barr virus shuttle vectors in human cells. Journal of Virology. ,vol. 62, pp. 3738- 3746 ,(1988) , 10.1128/JVI.62.10.3738-3746.1988
D Reisman, J Yates, B Sugden, A putative origin of replication of plasmids derived from Epstein-Barr virus is composed of two cis-acting components. Molecular and Cellular Biology. ,vol. 5, pp. 1822- 1832 ,(1985) , 10.1128/MCB.5.8.1822
Bernhard Hirt, Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology. ,vol. 26, pp. 365- 369 ,(1967) , 10.1016/0022-2836(67)90307-5
Susana de la Luna, Inmaculada Soria, Diego Pulido, Juan Ortín, Antonio Jiménez, Efficient transformation of mammalian cells with constructs containing a puromycin-resistance marker Gene. ,vol. 62, pp. 121- 126 ,(1988) , 10.1016/0378-1119(88)90585-9
T. Kitamura, M. Onishi, S. Kinoshita, A. Shibuya, A. Miyajima, G. P. Nolan, Efficient screening of retroviral cDNA expression libraries. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 92, pp. 9146- 9150 ,(1995) , 10.1073/PNAS.92.20.9146
Dan R. Rawlins, Gregory Milman, S.Diane Hayward, Gary S. Hayward, Sequence-specific DNA binding of the Epstein-Barr virus nuclear antigen (EBNA-1) to clustered sites in the plasmid maintenance region Cell. ,vol. 42, pp. 859- 868 ,(1985) , 10.1016/0092-8674(85)90282-X