Speciation of tissue and cellular iron with on-line detection by inductively coupled plasma-mass spectrometry.

摘要: Abstract Iron accumulating to excess in tissues of humans and animal models occurs mainly as complexes with transferrin, ferrin, ferritin, other hemoproteins, insoluble hemosiderin particles. To determine the distribution Fe amongst these molecular species, we have used inductively coupled plasma-mass spectrometry a means on-line, isotope-specific detection for their liquid chromatographic separation. The stable isotope 57 is suitable monitoring content each fraction, its availability at high isotopic enrichment makes it an attractive choice tracer studies when use radioisotope undesirable, e.g., human subjects. system offers advantages sensitivity (detection limits parts per billion range), wide dynamic range (linearity calibration curve over several orders magnitude), on-line analysis facilitating real-time evaluation separation, addition isotopespecific information. distributions healthy rat livers, liver heart tissue from Fe-loaded subjects, hepatocyte cultures are reported. ferritin:hemosiderin ratio samples shown be indicator degree loading correlates well that determined by Zeemancorrected electrothermal atomic absorption alternative detection.