[24] Monoclonal antibodies to transferrin receptor and assay of their biological effects
作者： Ian S. Trowbridge , Jayne F. Lesley , Derrick Domingo , Roberta Schulte , Carol Sauvage
DOI: 10.1016/0076-6879(87)47117-6
关键词:
摘要: Publisher Summary This chapter describes the derivation of monoclonal antibodies against transferrin receptor and assay their biological effects. Antibodies that block function have proved to be useful in dissecting role receptors iron uptake cell growth. Monoclonal human, murine, raw cells been obtained. With one exception, all these were derived from fusions spleen mice or rats immunized with viable whole xenogeneic cells, usually cultured hematopoietic lines activated lymphocytes. For most types, is major source iron, transferrin-mediated takes place via receptor-mediated endocytosis. After release under acid conditions within endosomal membrane compartment, apotransferrin remains bound recycled back surface. The expression on growing obligatory requirement for suggests this transport system itself are important
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