TGF-β1 induced MMP-9 expression in HNSCC cell lines via Smad/MLCK pathway
作者： Sariya Nuchanardpanit Sinpitaksakul , Atiphan Pimkhaokham , Neeracha Sanchavanakit , Prasit Pavasant
DOI: 10.1016/J.BBRC.2008.04.128
关键词: Head and neck squamous-cell carcinoma 、 Extracellular matrix 、 Signal transduction 、 Phosphorylation 、 Myosin light-chain kinase 、 Biology 、 Cell biology 、 SMAD 、 Cell culture 、 Transforming growth factor
摘要: Abstract Matrix metalloproteinase-9 (MMP-9) plays roles in cancer progression by degrading the extracellular matrix and basement membrane. Many growth factors including Transforming factor-beta1 (TGF-β1) could induce MMP-9 expression. We demonstrated that TGF-β1 induced mRNA protein human head neck squamous cell carcinoma lines. Application of TGF-β receptor type I inhibitor (SB505124) reduced expression markedly. Whilst, Myosin light chain kinase (MLCK) reduce level secreted both supernatants lysate but not mRNA. These suggested MLCK might regulate post-transcriptionally. SB505124 siRNA Smad2/3 phosphorylation myosin (MLC) MLC is downstream to TβRI/Smad2/3 signaling pathway. In conclusion, these results describe a novel mechanism for potentiation via Smad MLCK.
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