Effect of the chemical composition of organic extracts from environmental and industrial atmospheric samples on the genotoxicity of polycyclic aromatic hydrocarbons mixtures

摘要: Particulate organic matter (PM) present in the atmosphere is a complex mixture of chemicals like polycyclic aromatic hydrocarbons (PAH) that may exert adverse health effects including respiratory and cardiovascular disturbances cancer. In this study, airborne samples from environmental or industrial areas exhibiting different physicochemical composition were compared for their capacities to induce DNA damage human hepatocytes HepG2. strand breaks adducts formed by benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide (BPDE), most reactive metabolite carcinogenic benzo[a]pyrene (B[a]P), measured with comet assay high-performance liquid chromatography (HPLC)/mass spectrometry, respectively. Cells exposed extracted PM. Experiments performed either at constant concentration B[a]P concentrations corresponding fixed air volumes. Results show extracts tend produce more diol epoxide-N 2-2′-deoxyguanosine (BPDE-N 2-dGuo) than breaks, whereas opposite was observed extracts. The chemical significantly impacts nature levels damage. amount interaction other contaminants need be considered explain formation These results emphasize use vitro tests as promising complementary tools widely used toxic equivalent factor (TEF) approach order assess hazards related exposure general population.