The enhanced antitumor effects of biodegradable cationic heparin-polyethyleneimine nanogels delivering HSulf-1 gene combined with cisplatin on ovarian cancer

摘要: HSulf-1 (heparan sulfate 6-O-endosulfatase 1), a commonly downregulated gene in the majority of ovarian cancer cell lines, has been identified to play an important role regulating tumorigenesis. Our previous studies demonstrated that could inhibit angiogenesis and tumorigenesis vivo. The employment polymeric nanoparticles deliver functional holds much promise as effective therapeutic strategy against cancer. To develop more therapy, this study, we investigated antitumor effect heparin-polyethyleneimine (HPEI) nanogels delivering combined with cisplatin (DDP) on Expression vitro vivo was determined by reverse transcription polymerase chain reaction (RT-PCR) western blot analysis. A SKOV3 intraperitoneal carcinomatosis model nude mice established assess efficacy. Mice were treated NS, pEP/HPEI complexes, pHSulf-1/HPEI DDP or plus DDP, respectively. Intraperitoneal tumors weighed. Antiangiogenic evaluated CD31 immunostaining alginate-encapsulate tumor assay. Detection proliferative cells apoptotic tissues performed Ki-67 staining TUNEL Stable expression detected groups. combination complexes exhibited enhanced activity, compared monotherapy alone (P<0.01). therapy exerted significant activity through antiangiogenesis, induction apoptosis suppression proliferation. Collectively, these observations provide evidence HPEI may have promising application human