Increased NG2(+) glial cell proliferation and oligodendrocyte generation in the hypomyelinating mutant shiverer.

摘要: Glial cells that express the NG2 proteoglycan (NG2+ cells) are considered to be oligodendrocyte progenitors (OPCs) in central nervous system (CNS), based on their ability give rise mature oligodendrocytes vitro. To understand how dysmyelinated conditions influence OPC proliferation and differentiation, we studied differentiation of NG2+ OPCs vivo shiverer mutant (shi), which do not form compact myelin due a deletion basic protein gene. Acute bromodeoxyuridine (BrdU) labeling studies revealed 4- 6-fold increase cell shi spinal cord between postnatal day18 (P18) P60, most BrdU+ were after P18. The increased was accompanied by 2-fold number oligodendrocytes. Survival following single injection BrdU at P18 decline BrdU+/NG2+ with concomitant over time, suggesting proliferated had differentiated into generated longer period time persisted than wild type cord. These findings suggest new continue enhanced progenitor cells. © 2004 Wiley-Liss, Inc.