Method for preparing polynucleotide sequences and uses thereof
作者： Milton H. Werner , Vineet Gupta
DOI:
关键词: Nuclear magnetic resonance spectroscopy 、 Combinatorial chemistry 、 Dna duplex 、 Stereochemistry 、 DNA synthesis 、 Polynucleotide 、 Sequence (biology) 、 Single product 、 Chemistry 、 DNA 、 Macromolecule
摘要: Methods are described for the preparation of quantities preselected polynucleotide sequences in purified form. The method is independent length or sequence, and can be used to prepare multi-milligram of, example, single length, isotope-enriched duplex DNA following a single-step chromatographic purification desired product. present invention allows preparative scale synthesis yielding product length. application NMR spectroscopy provides opportunity identify biologically relevant interactions between other macromolecules, assist identification development agents having therapeutic utility as consequence promotion antagonism these interactions.
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