Inhibition of osteopontin reduces liver metastasis of human pancreatic cancer xenografts injected into the spleen in a mouse model.
作者： Keisuke Ohno , Hidefumi Nishimori , Takahiro Yasoshima , Kenjiro Kamiguchi , Fumitake Hata
DOI: 10.1007/S00595-009-4082-X
关键词: Metastasis 、 Osteopontin 、 Spleen 、 Cancer research 、 Cell culture 、 Transfection 、 CA19-9 、 Oncology 、 Medicine 、 Transplantation 、 Pancreatic cancer 、 Internal medicine
摘要: Pancreatic cancer is associated with the poorest prognosis of any digestive due to high incidence liver metastasis. This study evaluated possibility that osteopontin (OPN) RNA interference (RNAi) and anti-OPN antibody (Ab) could have antimetastatic effects. The differential gene expression was measured in a parental cell line, HPC-3, an established highly metastatic HPC-3H4. investigated effect OPN RNAi Ab on ability HPC-3H4 liver. An RNAi-expressing vector introduced into cells (HPC-3H4/miOPN), which production reduced level HPC-3 cells. Finally, suppress metastasis investigated. Osteopontin upregulated 11.1-fold comparison HPC-3. rate HPC-3H4/miOPN significantly 25% 100% control HPC-3H4/miNeg (P < 0.01). group given 50%. had remarkable inhibitory effects against by pancreatic line.
springer.com
sci-hub.se 参考文章(34)
T Yasoshima, H Ura, R Denno, T Shishido, K Hirata, K Yamaguchi, Establishment and characterization of a human gastric carcinoma cell line that is highly metastatic to lymph nodes. Journal of Experimental & Clinical Cancer Research. ,vol. 19, pp. 113- 120 ,(2000)
Hiroki Nomura, Hidefumi Nishimori, Takahiro Yasoshima, Fumitake Hata, Hiroshi Tanaka, Futoshi Nakajima, Toshio Honma, Jun Araya, Kenjiro Kamiguchi, Hiroshi Isomura, Noriyuki Sato, Ryuichi Denno, Koichi Hirata, A new liver metastatic and peritoneal dissemination model established from the same human pancreatic cancer cell line: analysis using cDNA macroarray. Clinical & Experimental Metastasis. ,vol. 19, pp. 391- 399 ,(2002) , 10.1023/A:1016370532618
A. Miyauchi, J. Alvarez, E.M. Greenfield, A. Teti, M. Grano, S. Colucci, A. Zambonin-Zallone, F.P. Ross, S.L. Teitelbaum, D. Cheresh, Recognition of osteopontin and related peptides by an alpha v beta 3 integrin stimulates immediate cell signals in osteoclasts. Journal of Biological Chemistry. ,vol. 266, pp. 20369- 20374 ,(1991) , 10.1016/S0021-9258(18)54932-2
David T. Denhardt, Ann F. Chambers, Sylvia M. Wilson, Ann Marie Craig, Elke I. Behrend, Reduced Malignancy of ras-transformed NIH 3T3 Cells Expressing Antisense Osteopontin RNA Cancer Research. ,vol. 54, pp. 832- 837 ,(1994)
Y Wu, D T Denhardt, S R Rittling, Osteopontin is required for full expression of the transformed phenotype by the ras oncogene British Journal of Cancer. ,vol. 83, pp. 156- 163 ,(2000) , 10.1054/BJOC.2000.1200
N. Shijubo, T. Segawa, T. Uede, M. Hirosawa, A. ′ Imada, S. Kon, S. Abe, M. Maeda, Vascular endothelial growth factor and osteopontin in stage I′ lung adenocarcinoma. Collected papers from Institute for Genetic Medicine Hokkaido University. ,vol. 1, pp. 139- 143 ,(1999)
Ralph H. Hruban, Neal S. Fedarko, Anirban Maitra, Jens Koopmann, Charles J. Yeo, Alka Jain, Ayman Rahman, Michael Goggins, Christine Iacobuzio-Donahue, Evaluation of Osteopontin as Biomarker for Pancreatic Adenocarcinoma Cancer Epidemiology, Biomarkers & Prevention. ,vol. 13, pp. 487- 491 ,(2004)
Takayuki Shishido, Takahiro Yasoshima, Koichi Hirata, Ryuichi Denno, Mitsuhiro Mukaiya, Hideki Ura, Koji Yamaguchi, Satoshi Kawaguchi, Noriyuki Sato, Establishment and characterization of human pancreatic carcinoma lines with a high metastatic potential in the liver of nude mice. Surgery Today. ,vol. 29, pp. 519- 525 ,(1999) , 10.1007/BF02482346
Alan B. Tuck, Charulata Hota, Ann F. Chambers, Osteopontin(OPN)-induced increase in human mammary epithelial cell invasiveness is urokinase (uPA)-dependent. Breast Cancer Research and Treatment. ,vol. 70, pp. 197- 204 ,(2001) , 10.1023/A:1013095329825
Yukihiro Yokoyama, Yuji Nimura, Masato Nagino, Advances in the treatment of pancreatic cancer: Limitations of surgery and evaluation of new therapeutic strategies Surgery Today. ,vol. 39, pp. 466- 475 ,(2009) , 10.1007/S00595-008-3904-6