Structural and functional domains of the myb oncogene: requirements for nuclear transport, myeloid transformation, and colony formation.

摘要: The v-myb oncogene of avian myeloblastosis virus causes acute myelomonocytic leukemia in vivo and transforms only myeloid cells vitro. Its product, p48v-myb, is a nuclear protein unknown function. To determine structure-function relationships for this protein, we constructed series deletion mutants v-myb, expressed them retroviral vectors, studied their biochemical biological properties. We used these to identify two separate domains p48v-myb which had distinct roles its accumulation the cell nucleus. showed that viral sequences normally encode both termini were dispensible transformation. In contrast, copies highly conserved amino-terminal repeat required also identified carboxyl-terminal domain was growth v-myb-transformed myeloblasts soft agar but not morphological