Growth factor gene activation and clonal heterogeneity in an autostimulatory myeloid leukemia.

摘要: Cell lines were isolated from an in vivo-passaged myelomonocytic leukemia, WEHI-274, that arose a mouse infected with the Abelson leukemia virus-Moloney virus complex. Clones vitro presence or absence of source hemopoietic growth factor, interleukin-3 (IL-3), and divisible into three distinct classes. All classes leukemogenic vivo. In vitro, class I clone grew slowly at low cell density but responded increased rate to IL-3, granulocyte-macrophage colony-stimulating factor (GM-CSF), autoconditioned medium. Supernatants these cultures contained biological, biochemical, antigenic properties IL-3. Class II clones better densities than did also GM-CSF, autoconditional medium produced GM-CSF rather contrast, III died all unless exogenous IL-3 was added. Moreover, they no autostimulatory factors. clones, one allele respective gene is rearranged, each case, grossly abnormal RNA transcripts rearranged are present. Neither rearrangements nor detected clones. exhibited common rearrangement c-myb gene, which suggested derived ancestral cell. These experiments demonstrate two independent events involved progression single disease.