First detection of Echinococcus granulosus G1 and G7 in wild boars (Sus scrofa) and red deer (Cervus elaphus) in Romania using PCR and PCR-RFLP techniques.
作者： D. Onac , A. Győrke , M. Oltean , R. Gavrea , V. Cozma
DOI: 10.1016/J.VETPAR.2012.11.044
关键词: Echinococcus 、 Wild boar 、 Genotype 、 Liver parenchyma 、 Cervus elaphus 、 Restriction fragment length polymorphism 、 Veterinary medicine 、 Biology 、 Echinococcus granulosus
摘要: The epidemiological status of CE in our country places Romania into the top European countries and among first worldwide. Two hundred ninety wild animals (267 boars, 21 red deer 2 mouflons) hunted a private ground from Bihor county western were subject necropsy January 2012.Out 290 animals, 35 positive during for hydatic cysts these, 33 boars had cystsonly liver parenchyma. This paper presents identification Echinococcus granulosus G1 cervids (100%). In it identified (45.5%) G7 genotypes (39.4%). mouflons free cysts. Our resultsconfirmthat sheep strain (G1) is predominant genotype, which occurs, followed closely by pig (G7) that wildlife reservoirs should be taken consideration management disease.
elsevier.com
sci-hub.se 参考文章(13)
Adriano Casulli, Maria Interisano, Tamas Sreter, Lidia Chitimia, Zvezdelina Kirkova, Giuseppe La Rosa, Edoardo Pozio, Genetic variability of Echinococcus granulosus sensu stricto in Europe inferred by mitochondrial DNA sequences. Infection, Genetics and Evolution. ,vol. 12, pp. 377- 383 ,(2012) , 10.1016/J.MEEGID.2011.12.014
Roxana Moldovan, Adriana Maria Neghina, Crenguta Livia Calma, Iosif Marincu, Raul Neghina, Human cystic echinococcosis in two south-western and central-western Romanian counties: a 7-year epidemiological and clinical overview. Acta Tropica. ,vol. 121, pp. 26- 29 ,(2012) , 10.1016/J.ACTATROPICA.2011.10.003
E. MOKS, I. JÕGISALU, H. VALDMANN, U. SAARMA, First report of Echinococcus granulosus G8 in Eurasia and a reappraisal of the phylogenetic relationships of 'genotypes' G5-G10. Parasitology. ,vol. 135, pp. 647- 654 ,(2008) , 10.1017/S0031182008004198
J. M. Bart, S. Morariu, J. Knapp, M. S. Ilie, M. Pitulescu, A. Anghel, I. Cosoroaba, R. Piarroux, Genetic typing of Echinococcus granulosus in Romania. Parasitology Research. ,vol. 98, pp. 130- 137 ,(2006) , 10.1007/S00436-005-0015-9
Josephine Bowles, Donald P McManus, None, Rapid discrimination of Echinococcus species and strains using a polymerase chain reaction-based RFLP method. Molecular and Biochemical Parasitology. ,vol. 57, pp. 231- 239 ,(1993) , 10.1016/0166-6851(93)90199-8
K Daniel Mwambete, F Ponce-Gordo, C Cuesta-Bandera, Genetic identification and host range of the Spanish strains of Echinococcus granulosus. Acta Tropica. ,vol. 91, pp. 87- 93 ,(2004) , 10.1016/J.ACTATROPICA.2004.04.001
R. Sobrino, L. M. Gonzalez, J. Vicente, D. Fernández de Luco, T. Garate, C. Gortázar, Echinococcus granulosus (Cestoda, Taeniidae) in the Iberian wolf Parasitology Research. ,vol. 99, pp. 753- 756 ,(2006) , 10.1007/S00436-006-0229-5
Raul Neghina, Adriana M. Neghina, Iosif Marincu, Ioan Iacobiciu, Epidemiology and history of human parasitic diseases in Romania Parasitology Research. ,vol. 108, pp. 1333- 1346 ,(2011) , 10.1007/S00436-011-2256-0
L.M. González, K. Daniel-Mwambete, E. Montero, M.C. Rosenzvit, D.P. McManus, T. Gárate, C. Cuesta-Bandera, Further molecular discrimination of Spanish strains of Echinococcus granulosus. Experimental Parasitology. ,vol. 102, pp. 46- 56 ,(2002) , 10.1016/S0014-4894(02)00146-7
A. LAVIKAINEN, M. J. LEHTINEN, T. MERI, V. HIRVELÄ-KOSKI, S. MERI, Molecular genetic characterization of the Fennoscandian cervid strain, a new genotypic group (G10) of Echinococcus granulosus Parasitology. ,vol. 127, pp. 207- 215 ,(2003) , 10.1017/S0031182003003780