Immune Response to Individual Maedi-Visna Virus gag Antigens

摘要: The lesions caused by maedi-visna virus (MVV) are known to be immune mediated with a presumed contribution the response viral antigens. However, very little is about T-cell individual proteins. We have therefore expressed three gag antigens of MVV strain EV1 (p16, p25, and p14) in bacterial expression system used purified recombinant proteins analyze antibody CD4+ MVV. Plasma samples were taken from sheep after 1 year infection titers for antibodies these determined indirect enzyme-linked immunosorbent assays as follows: anti-p25 antibody, 1:400 >1:3,200; anti-p16 1:3,200; anti-p14 1:<100 1:3,200. When induction was followed over time postinfection (p.i.), positive seen day 24 p.i., 45 lastly 100 p.i. proliferative responses all detected persistently infected peripheral blood lymphocytes. raise lines sheep. These shown specific antigen on macrophages. restricted major histocompatibility complex class II HLA-DR so due T All may play role immune-mediated lesion formation disease presentation macrophages lesions.