Analysis of antibody responses to phenotypically distinct lentiviruses.

摘要: To define the immune responses against phenotypically and pathogenically distinct lentiviruses, we used an immunoblotting assay to study antibodies viral proteins of ovine lentivirus (OvLV) in 16 experimentally 12 naturally infected sheep. Two phenotypes OvLV were infect lambs: strain 85/34, a "rapid/high" isolate which rapidly induced lysis primary macrophage cultures replicated relatively high titers, strains 84/28 85/14, "slow/low" isolates slowly progressive syncytia with minimal vitro only low titers same cell type. Serum four major structural proteins, gp105, p25, p16, p14, detected. In longitudinal lambs, antibody p25 (major gag protein) usually appeared first (average, about 3 weeks postinoculation [p.i.]) was followed 2 by gp105 almost simultaneously. Six animals did not develop anti-p14 time necropsy at 9 29 p.i. 10 lambs developed p14 had transiently from 8 or 13 lost it 21 22 contrast, other three remained fairly constant until necropsy. There differences two OvLV. Seven (70%) inoculated rapid/high whereas 17% slow/low this protein. study, no decline observed activity any specific such as that occurs anti-p24 human immunodeficiency virus infection, except case lambs. significant final blood samples between virus- virus-infected groups. However, group fivefold-higher geometric mean titer anti-env product (gp 105) than (P