Immune response to recombinant visna virus Gag and Env precursor proteins synthesized in insect cells.
作者： Björg Rafnar , Gregory J Tobin , Kunio Nagashima , Matthew A Gonda , Eggert Gunnarsson
DOI: 10.1016/S0168-1702(97)00141-X
关键词:
摘要: Abstract Two different recombinant visna virus (VV) gag -baculoviruses were constructed for the expression of precursor VV Gag in insect cells. Both viruses expressed proteins migrating on SDS–PAGE at predicted rate precursor, Pr50 . However, differences seen morphology virus-like particles produced. Monoclonal antibody directed against capsid protein (p25) and sera from sheep infected with ovine lentiviruses reacted to both 50-kDa proteins. A env -baculovirus was constructed, substituting sequences encoding signal peptide Env murine IFN- γ analogue. Sera lentivirus immunoblots two approximately 100 200 kDa found plasma membrane cells -recombinant virus. Sheep immunized either or developed high titers ELISA. The serum ascitic fluid mice native processed immunoblots, whereas gp135 a putative oligomer gp135. responded specifically by lymphocyte proliferation vitro.
elsevier.com
researchgate.net 
sci-hub.se 参考文章(53)
T. C. McGuire, D. S. Adams, G. C. Johnson, D. D. Barbee, J. R. Gorham, P. Klevjer-Anderson, Acute arthritis in caprine arthritis-encephalitis virus challenge exposure of vaccinated or persistently infected goats. American Journal of Veterinary Research. ,vol. 47, pp. 537- 540 ,(1986)
Maedi-visna and related diseases. Kluwer Academic Publishers. ,(1989) , 10.1007/978-1-4613-1613-8
Norman L. Letvin, Jean Claude Gluckman, color plastes, figures, tables, Animal models of HIV and other retroviral infections : Karger. ,(1993)
G. Georgsson, Maedi - Visna. Pathology and Pathogenesis Developments in veterinary virology (USA). pp. 19- 54 ,(1990) , 10.1007/978-1-4613-1613-8_3
Lois K. Miller, Verne A. Luckow, David R. O'Reilly, Baculovirus expression vectors: a laboratory manual. Baculovirus expression vectors: a laboratory manual.. ,(1992)
O Kajikawa, M D Lairmore, J C DeMartini, Analysis of antibody responses to phenotypically distinct lentiviruses. Journal of Clinical Microbiology. ,vol. 28, pp. 764- 770 ,(1990) , 10.1128/JCM.28.4.764-770.1990
Robert Vigne, Pierre Filippi, Gilles Quérat, Nicole Sauze, Christian Vitu, Pierre Russo, Pierre Delori, Precursor Polypeptides to Structural Proteins of Visna Virus Journal of Virology. ,vol. 42, pp. 1046- 1056 ,(1982) , 10.1128/JVI.42.3.1046-1056.1982
G J Tobin, R C Sowder, D Fabris, M Y Hu, J K Battles, C Fenselau, L E Henderson, M A Gonda, Amino acid sequence analysis of the proteolytic cleavage products of the bovine immunodeficiency virus Gag precursor polypeptide. Journal of Virology. ,vol. 68, pp. 7620- 7627 ,(1994) , 10.1128/JVI.68.11.7620-7627.1994
L M Bhaduri, O Narayan, J E Clements, J Stanley, Topographical rearrangements of visna virus envelope glycoprotein during antigenic drift. Journal of Virology. ,vol. 61, pp. 1019- 1028 ,(1987) , 10.1128/JVI.61.4.1019-1028.1987
Gregory J. Tobin, Jane K. Battles, Lynn Rasmussen, Matthew A. Gonda, [15] Assembly of recombinant retroviral gag precursors into pseudovirions in the baculovirus-insect cell expression system Methods in Molecular Genetics. ,vol. 7, pp. 237- 253 ,(1995) , 10.1016/S1067-2389(06)80047-6