Detection of antibodies to caprine arthritis-encephalitis virus using recombinant gag proteins

摘要: The coding sequences of the core proteins p17 and p28 caprine arthritis-encephalitis virus (CAEV) were amplified using polymerase chain reaction cloned into plasmid expression vector p-GEX-2T. Both expressed as fusion with glutathione S-transferase. recombinant affinity purified from induced bacterial lysates glutathione-agarose beads. used in an enzyme-linked immunosorbent assay (ELISA) to detect antibodies against CAEV goat sera milk samples. Three different ELISA tests developed based on p17, or combination these two (p17+p28). A comparison was made whole particles agar immunodiffusion test (AGID). Sera conflicting results examined by Western blotting. There a high correlation between p17+p28 ELISA, estimated κ value 0.92. Only 72–75% that tested positive AGID. Antibodies detected significantly more animals when serum samples compared Based time materials required prepare reagents, less expensive than ELISA.