Design and Characterization of a Sensorized Microfluidic Cell-Culture System with Electro-Thermal Micro-Pumps and Sensors for Cell Adhesion, Oxygen, and pH on a Glass Chip

摘要: We combined a multi-sensor glass-chip with microfluidic channel grid for the characterization of cellular behavior. The was imprinted in poly-dimethyl-siloxane. Mouse-embryonal/fetal calvaria fibroblasts (MC3T3-E1) were used as model system. Thin-film platinum (Pt) sensors respiration (amperometric oxygen electrode), acidification (potentiometric pH electrodes) and cell adhesion (interdigitated-electrodes structures, IDES) allowed us to monitor cell-physiological parameters well cell-spreading Two on-chip electro-thermal micro-pumps (ETμPs) permitted induction medium flow system, e.g., mixing drug delivery. glass-wafer technology ensured microscopic observability culture. Connecting Pt structures passivated by 1.2 μm layer silicon nitride (Si3N4). Thin Si3N4 layers (20 nm or 60 nm) sensitive material electrodes. These electrodes showed linear behavior range from 4 9, sensitivity up 39 mV per step. circular sensor area 78.5 μm2. Their 100 pA 1% increase 0% 21% (air saturated). different IDES geometries 30- 50-μm finger spacings comparable sensitivities detecting proliferation rate MC3T3 cells. cells cultured 11 days vitro test biocompatibility, microfluidics electric our system under standard laboratory conditions.